Loss of <i>Myostatin</i> Shapes the Transcriptomic and Epigenetic Landscapes Across Multiple Muscle Types in Cattle.

ABSTRACT: Myostatin (MSTN) is a critical regulator of muscle development. This study aimed to investigate the transcriptional and epigenetic mechanisms by which MSTN gene editing affects skeletal, cardiac, and smooth muscle function in cattle. The results showed that the MSTN gene-edited (MT) cattle skeletal muscle exhibited significantly larger myofiber cross-sectional areas (p = 0.049), accompanied by reduced shear force (p = 0.044), cooking loss rate (p = 0.0029), and pH (p = 0.014). Transcriptomic and whole-genome bisulfite sequencing (WGBS) revealed distinct expression and methylation patterns across muscle types. Notably, axon guidance signaling was identified as a shared enriched pathway in both transcriptional and CG/CHG/CHH methylation

SUBMITTER: Hai C 

PROVIDER: S-EPMC12192037 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature