Genetic Transformation of the Model Quorum Sensing Bacterium <i>Vibrio campbellii</i> by Electroporation.

ABSTRACT:

Background

The marine bacterium Vibrio campbellii has been a model system for the study of bacterial quorum sensing and is increasingly recognized as a formidable aquatic animal pathogen. While genetically tractable, the study of this species in basic and applied research still relies upon laborious and time-consuming conjugation methods for plasmid DNA transformation.

Methods

In this study, we developed an electroporation protocol using the most studied strain of this species, V. campbellii ATCC BAA-1116. An electroporation efficiency of up to 3 × 10⁴ CFU/μg DNA was demonstrated using derived parameters (10 kV/cm, 400 Ω, 25 μF), which took cell growth phase at harvest, plasmid DNA amount, and recovery conditions into account. The electroporation pro

SUBMITTER: Tschirhart T 

PROVIDER: S-EPMC12192358 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature