Cytogenetic and Molecular Characterization of <i>Sphaerophoria rueppellii</i> (Diptera, Syrphidae).

ABSTRACT: Sphaerophoria rueppellii is a Palearctic hoverfly widely used as a native biocontrol agent against aphid pests in Mediterranean agroecosystems. In this study, we present a cytogenetic analysis and characterization of the mitochondrial genome of this species. Chromosomal preparations, obtained from third-instar larvae, were used for conventional staining, DAPI staining and C-banding techniques, and major ribosomal DNA (rDNA) location by fluorescence in situ hybridization (FISH). Karyotype analysis revealed a diploid number of 2n = 10, with heterochromatic blocks in the pericentromeric regions of all autosomes and rDNA clusters on both sex chromosomes. The complete mitochondrial genome (16,605 bp) was sequenced and annotated using next-generation sequencing and assembly pipelines. It

SUBMITTER: Lorite P 

PROVIDER: S-EPMC12193307 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature