Exceptional Heme Tolerance in <i>Serratia plymuthica</i>: Proteomic Insights into Oxidative Stress Adaptation in the <i>Aedes aegypti</i> Midgut.

ABSTRACT: Serratia plymuthica, isolated from the midgut of Aedes aegypti, displays remarkable resilience to hemin, a toxic hemoglobin byproduct generated during blood digestion. This study explores its proteomic adaptations under oxidative stress induced by 5 mM hemin, mimicking midgut conditions. Growth assays demonstrated that S. plymuthica tolerated hemin concentrations ranging from 5 µM to 1 mM, reaching the stationary phase within approximately 10 h. Colonies exhibited morphological changes-darkened peripheries and translucent halos-suggesting heme accumulation and detoxification. Label-free quantitative proteomics identified 436 proteins, among which 28 were significantly upregulated-including universal stress proteins (USPs), ABC transporters, and flavodoxin-while 54 were

SUBMITTER: Machado SDH 

PROVIDER: S-EPMC12193951 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature