Isolation of <i>Bacillus amyloliquefaciens</i> D39 and Identification of Its Antimicrobial Proteins Active Against Chestnut Blight.

ABSTRACT: Chestnut blight, caused by Cryphonectria parasitica (Murrill) M.E. Bar, is a destructive fungal disease threatening chestnut cultivation and production. In response to the limitations of chemical control, biological control using antagonistic microbes has gained increasing attention. A rhizosphere-derived bacterium, strain D39, was isolated from healthy chestnut trees and identified as Bacillus amyloliquefaciens based on morphological characteristics and the phylogenetic analysis of 16S rRNA and gyrA genes. The antifungal activity of strain D39 against C. parasitica was evaluated using dual-culture and double-layer Oxford cup assays. The strain exhibited broad-spectrum and stable antagonistic effects and harbored five key genes associated with antimicrobial comp

SUBMITTER: Deng T 

PROVIDER: S-EPMC12195296 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature