Synergistic Engineering of the Twin-Arginine Translocation (Tat) Pathway and Membrane Capacity Enhances Extracellular Production of Amylosucrase in <i>Bacillus licheniformis</i>.

ABSTRACT: Amylosucrase (AS) is a highly versatile enzyme with significant potential for industrial applications, including functional food production and glycosylation of bioactive compounds. However, its large-scale production is hampered by low secretion efficiency in microbial hosts. This study focuses on engineering the twin-arginine translocation (TAT) pathway and optimizing membrane resource allocation in Bacillus licheniformis to enhance the extracellular production of Neisseria polysaccharea amylosucrase (NpAS). The investigation integrates three targeted strategies: optimizing the hydrophobic region adjacent to the TAT signal peptide, modifying TAT translocases via site-directed mutagenesis, and improving inter-pathway membrane resource redistribution by deleting non-essential

SUBMITTER: Wang C 

PROVIDER: S-EPMC12195555 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature