Interaction Between <i>Enterococcus faecalis</i> and <i>Fusobacterium nucleatum</i> Regulated Macrophage Transcriptional Profiling and Reprogrammed Cellular Immune and Metabolic Response.

ABSTRACT: Refractory apical periodontitis (RAP), a persistent infection after root canal treatment, still has no effective treatment. Enterococcus faecalis (E. faecalis) and Fusobacterium nucleatum (F. nucleatum) are frequently detected in the lesion. We previously found that coaggregation altered gene expression of E. faecalis and F. nucleatum and promoted immune evasion by suppressing pro-inflammatory cytokine secretion of macrophages (Mφs) while sustaining low-grade inflammation. In this study, we further investigated the synergistic effect of coaggregated E. faecalis and F. nucleatum on modulating Mφ immune and metabolic responses. Using transmission electron microscope, flow cytometry, RNA-seq and functional assays, we demonstrated that co

SUBMITTER: Liang J 

PROVIDER: S-EPMC12195608 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature