Whole-Genome Sequence-Based Diversity of <i>Mycobacterium tuberculosis</i> Strains Isolated from a Central Western Region of Mexico.

ABSTRACT: Tuberculosis remains a significant health issue in Mexico, which has one of the highest incidence rates in the Americas. This study aimed to analyze the circulating sublineages, spoligotypes, drug resistance, and transmission patterns of Mycobacterium tuberculosis in Mexico's Central Western region using whole-genome sequencing. Seventy-seven Mycobacterium tuberculosis strains underwent phenotypic drug susceptibility testing via MGIT. Genotypic resistance was assessed with TB-Profiler and Mykrobe, while phylogenetic relationships were reconstructed using Snippy and RaxML. SpoTyping identified circulating SITs and families, with a 5-SNP threshold defining genomic transmission clusters. The predominant sublineages were 4.1.1.3 (X-type, n = 19) and 4.1.2.1 (LAM, n

SUBMITTER: Negrete-Paz AM 

PROVIDER: S-EPMC12195799 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature