Genome Editing of the <i>NF-YA8</i> Gene Modifies Tomato Plant Architecture and Fruit Traits.

ABSTRACT: Genome editing has revolutionized plant science, providing an unprecedented ability to precisely manipulate plant genomes. For this study, genome editing was utilized to target and modify the NF-YA8 transcription factor (TF) in tomato plants (Solanum lycopersicum L. var. Heinz 1706). The primary objective of this research was to introduce targeted mutations in a non-transgenic manner to the NF-YA8 gene, which encodes the alpha subunit of the Nuclear Factor-Y (NF-Y) heterotrimeric TF, and explore its potential for developing new and improved tomato varieties. Through the transient expression of custom-engineered zinc finger nucleases (ZFNs) in tomato seeds, mutations were successfully introduced in the target gene. The recovered mutant NF-YA8 coding sequences showed a s

SUBMITTER: Petrou N 

PROVIDER: S-EPMC12196555 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature