Quantitative Detection of Micro- and Nanoplastics (≥300 nm) in Human Urine Using Double-Shot Py-GC/MS with Internal Standard Calibration.

ABSTRACT: The rapid increase in plastic production and consumption has intensified research into human exposure to micro- and nanoplastics (MNPs) and their health effects. This study quantitatively assessed MNP internal exposure levels in non-invasive human samples, focusing on the four most common types of MNPs (≥300 nm): polyethylene terephthalate (PET), polypropylene (PP), low-density polyethylene (LDPE), and polystyrene (PS). Urine samples from 18 volunteers (4 males, 14 females) were analyzed using pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) with P(E-¹³C₂) as an internal standard. The study developed a straightforward yet effective analytical approach for quantifying MNPs in biological fluids. MNPs were detected in all urine samples, with concentrations rangi

SUBMITTER: Ji S 

PROVIDER: S-EPMC12197141 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature