Unraveling the Central Role of Global Regulator PprI in <i>Deinococcus radiodurans</i> Through Label-Free Quantitative Proteomics.

ABSTRACT:

Background

Deinococcus radiodurans, renowned for its exceptional resistance to radiation, provides a robust model for elucidating cellular stress responses and DNA repair mechanisms. Previous studies have established PprI as a key regulator contributing to radiation resistance through its involvement in DNA damage repair pathways, oxidative stress response, and metabolic regulation.

Methods

Building upon these foundations, our study employs label-free quantitative (LFQ) proteomics coupled with high-resolution mass spectrometry to systematically map pprI deletion protein networks by comparing the global proteomic profiles of pprI knockout and wild-type D. radiodurans strains.

Results

Under stringent screening criteria, we identified 719 sign

SUBMITTER: Zhu S 

PROVIDER: S-EPMC12197293 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature