Impaired Glycosylation of Gastric Mucins Drives Gastric Tumorigenesis and Serves as a Novel Therapeutic Target.

ABSTRACT:

Background & aims

Gastric cancer is often accompanied by a loss of mucin 6 (MUC6), but its pathogenic role in gastric carcinogenesis remains unclear.

Methods

Muc6 knockout (Muc6^-/-) mice and Muc6-dsRED mice were newly generated. Tff1Cre, Golph3^-/-, R26-Golgi-mCherry, Hes1^flox/flox, Cosmc^flox/flox, and A4gnt^-/- mice were also used. Histology, DNA and RNA, proteins, and sugar chains were analyzed by whole-exon DNA sequence, RNA sequence, immunohistochemistry, lectin-binding assays, and liquid chromatography-mass spectrometry analysis. Gastric organoids and cell lines were used for in vitro assays and xenograft experiments.

Results

Deletion of Muc6 in mice spontaneously causes pan-gastritis and invasive gastric cance

SUBMITTER: Arai J 

PROVIDER: S-EPMC12197564 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature