Deconvolution-Based Transcriptomic Analysis in the Hippocampus Reveals Cell Type-Specific Risk Genes and Pathways Associated With Depression and Suicide.

ABSTRACT:

Background

Major depressive disorder (MDD) is a prevalent mental health condition with the highest associated suicide risk among major psychiatric disorders. Understanding the molecular and cellular mechanisms is crucial for assessing the risk of MDD and associated suicide.

Methods

In this study, transcriptome-based deconvolution was applied to human postmortem hippocampal samples from nonpsychiatric control subjects (n = 29), subjects with MDD who died by means other than suicide (D-S; n = 15), and subjects with MDD who died by suicide (D+S; n = 29). A reference gene expression profile for deconvolution was established using single-nucleus RNA sequencing (snRNA-Seq) analysis. The proportions of various cell types were assessed using the MuSiC2 package,

SUBMITTER: Francis A 

PROVIDER: S-EPMC12197995 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature