Resistance of HEK-293 and COS-7 cell lines to oxidative stress as a model of metabolic response.

ABSTRACT: Oxidative stress (OS), arising from an imbalance between reactive oxygen species (ROS) production and antioxidant defenses, plays a pivotal role in cellular dysfunction and the pathogenesis of numerous diseases. This study evaluates the impact of oxidative stress induced by hydrogen peroxide on the metabolomic profiles of the human embryonic kidney (HEK-293) and African green monkey kidney (COS-7) cell lines. Viability (MTT) and free radical accumulation (DCF-DA) assays confirmed a dose-dependent cytotoxic effect of hydrogen peroxide, with COS-7 cells exhibiting greater resistance and producing lower levels of intracellular ROS compared to HEK-293. Metabolomic profiling was conducted using nuclear magnetic resonance spectroscopy (¹H NMR) to identify and quantify metabolic change

SUBMITTER: Sapeta-Nowinska M 

PROVIDER: S-EPMC12198024 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature