Improving recombinant protein secretion in <i>Aspergillus nidulans</i> by targeting the N-glycosylation machinery.

ABSTRACT: Filamentous fungi are cell factories traditionally used for enzyme production in various industrial sectors, including food and beverages, biopolymers, biofuels, and animal feed. Despite significant progress in optimizing enzyme production, challenges related to cost-effectiveness persist. Genes involved in the fungal secretory pathway have been modified to address productivity barriers, including post-translational modifications such as N-glycosylation of proteins. N-glycosylation can significantly affect protein stability, production yield, and functionality. This study investigated the isolated and combined deletion of genes involved in N-glycan assembly on protein production in Aspergillus nidulans. To test this hypothesis, we utilized CRISPR/Cas9 technology to knock out 14 gene

SUBMITTER: Gerhardt JA 

PROVIDER: S-EPMC12198042 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature