Genomic evolution and dissemination of non-conjugative virulence plasmid of ST65 carbapenem-resistant and hypervirulent <i>Klebsiella pneumoniae</i> strains in a Chinese hospital.

ABSTRACT:

Background

The global rise in infections caused by hypervirulent and carbapenem-resistant K. pneumoniae (CR-hvKp) represents a growing public health threat. This study investigates ST65 CR-hvKp strains, with a focus on their genomic attributes and the mechanisms underlying the transmission of non-conjugative virulence plasmids.

Methods

Two clinical K2-ST65 CR-hvKp isolates (P6 and P10) were identified. Plasmid conjugation experiments were performed to assess the dissemination of the virulence plasmid. Antimicrobial susceptibility testing and virulence assays, including serum resistance, siderophore production, and G. mellonella larvae infection models, were used to characterize resistance and virulence phenotypes. Comprehensive bioinformatic analyses were perfo

SUBMITTER: Tian D 

PROVIDER: S-EPMC12198223 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature