Aryl-alcohol oxidases: catalysis, diversity, structure-function and emerging biotechnological applications.

ABSTRACT: Aryl-alcohol oxidases (AAOs) are flavin-dependent enzymes of the glucose-methanol-choline (GMC) oxidoreductase superfamily that catalyze the oxidation of a broad range of activated primary alcohols into their corresponding aldehydes, generating hydrogen peroxide. While traditionally studied in wood-decaying fungi, AAOs have recently been identified in bacteria and arthropods, revealing unexpected structural and functional diversity. These enzymes display broad substrate promiscuity, with preferences shaped by differences in active-site architecture and physicochemical properties. Structural studies across kingdoms show a conserved GMC fold with specific adaptations in substrate-binding domains. Detailed mechanistic insights-particularly from the AAO from Pleurotus eryngii-suggest a consens

SUBMITTER: Cinca-Fernando P 

PROVIDER: S-EPMC12198266 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature