Iron regulatory pathways differentially expressed during Madurella mycetomatis grain development in Galleria mellonella.

ABSTRACT: Mycetoma is a chronic granulomatous infection of the subcutaneous tissue, most often caused by the fungal pathogen Madurella mycetomatis. Characteristic of the infection is the formation of grains. However, knowledge of the function and formation of the grain is limited. Here, we use a Galleria mellonella larvae infection model and transcriptomic profiling to identify processes associated with M. mycetomatis grain formation. Larvae were infected with M. mycetomatis and, after 4, 24, 72 and 168 h post-inoculation, RNA was extracted from larval content and sequenced. We found that 3498 G. mellonella and 136 M. mycetomatis genes were differentially expressed during infection. In particular, genes encoding proteins related to iron transport were highly expressed by both G. mellonella (transfer

SUBMITTER: Abugessaisa I 

PROVIDER: S-EPMC12198395 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature