High-throughput targeted amplicon screening tool for characterizing intrahost diversity in <i>Staphylococcus aureus</i> directly from sample.

ABSTRACT: A significant proportion of people are asymptomatic carriers of Staphylococcus aureus (SA), an important risk factor for the development of opportunistic infections. SA colonization is dynamic, appearing and disappearing, with strains evolving and potentially shifting in composition over time and between body sites. These changes make detection challenging, and the numerous potential sources of reintroduction from other people and even other body site reservoirs preclude efficient efforts to prevent transmission and spread. Identifying typical sources is therefore critical for mitigation. Whole-genome sequencing (WGS), ideally of multiple colonies from multiple body sites, is the gold standard for characterizing SA strains and confirming transmission. However, this is often too reso

SUBMITTER: Furstenau TN 

PROVIDER: S-EPMC12198441 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature