Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation.

ABSTRACT: Optogenetics offers the potential for mimicking complex spatiotemporal control of enzyme activity down to a subcellular resolution. However, most optogenetic approaches often face significant challenges in integrating multiple capabilities in a single tool applicable to a wide range of target proteins. Achieving precise control over ON/OFF kinetics, ensuring minimum leakiness in the dark, and demonstrating efficient performance in mammalian cells with subcellular precision are some of the most common challenges faced in this field. A promising solution lies in the application of rationally designed light-sensitive domains to allosterically control protein activity. Using that strategy, we generated an optogenetic method combining all the desired features. The approach involves the incorpor

SUBMITTER: Bansal T 

PROVIDER: S-EPMC12199233 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature