PABPN1 Couples the Polyadenylation and Translation of Maternal Transcripts to Mouse Oocyte Meiotic Maturation.

ABSTRACT: During oocyte meiosis, maternal transcript polyadenylation is crucial for regulating mRNA stability and translation, which are essential for oocyte maturation. Polyadenylate-binding protein nuclear 1 (PABPN1) plays a key role in regulating mRNA splicing and polyadenylation in somatic cells and growing oocytes. However, its potential function in regulating the meiotic maturation of fully grown oocytes remains unknown. This study reports that selective Pabpn1 knockout in growing mouse oocytes using Zp3-Cre do not affect folliculogenesis but prevented germinal vesicle breakdown in fully grown oocytes, impaired CDK1 activation, and resulted in abnormal spindle formation and chromosome misalignment. The results of poly(A)-inclusive full-length RNA isoform sequencing (PAIso-seq) and transcriptom

SUBMITTER: Dai XX 

PROVIDER: S-EPMC12199313 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature