The differential expression of MAGI2 in glomerulopathies and its application as a molecular discriminator of podocytopathies.

ABSTRACT:

Background

Podocyte dysfunction is central to various glomerular diseases, necessitating reliable biomarkers for early detection and diagnosis. This study investigates the regulatory mechanisms of membrane-associated guanylate kinase inverted 2 (MAGI2) and its potential as a biomarker for podocytopathies.

Methods

Using fluorescence confocal laser scanning microscopy and super-resolution structured illumination microscopy of immunostained tissue sections of murine, human, and zebrafish tissue we investigated the subcellular location of MAGI2 in the kidney. We assessed the differential regulation of MAGI2 in glomerular disease animal models, and isolated glomerular dedifferentiation using immunostainings and LC-MS/MS tandem mass spectrometry. With CRISPR-Cas9, we generated zeb

SUBMITTER: Siegerist F 

PROVIDER: S-EPMC12199530 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature