Transcriptome Analysis Suggested Striking Transition Around the End of Epiboly in the Gene Regulatory Network Downstream of the Oct4-Type POU Gene in Zebrafish Embryos.

ABSTRACT: Zebrafish pou5f3 encodes a Class V POU transcription factor, Pou5f3, which regulates various developmental processes, including neurogenesis and brain formation. In the current study, we attempted to comprehensively identify the Pou5f3 downstream genes around the end of epiboly, when the competence of the mid-hindbrain region to Pou5f3 suppression changes drastically, by the microarray method and a heat-inducible dominant-interference pou5f3 gene (en-pou5f3) that functionally suppresses pou5f3. At late epiboly and early somitogenesis stages, we identified genes whose expression was altered in en-pou5f3-induced embryos, revealing numerous genes regulated differently by Pou5f3 at the two stages. The validity of the microarray data was confirmed by whole mount in situ hybridization and quanti

SUBMITTER: Ikeda M 

PROVIDER: S-EPMC12199784 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature