Insights into rutin and quercetin biotransformations in ruminants revealed by molecular networking.

ABSTRACT: Quercetin and its glycoside rutin are flavonoids that are widely used as robust antioxidants and anti-inflammatory agents for improving animal health. These compounds are metabolized into various phenolic compounds that may have stronger antioxidant activity than their original forms. However, the quercetin and rutin biotransformation pathways in ruminants have not yet been fully elucidated. To identify these pathways, we used a molecular networking approach to determine the metabolite formation of rutin and quercetin. Five Shiba crossbred wethers (51.4 ± 7.4 kg BW) were used in a balanced crossover design and were assigned to 3 different groups: the quercetin (QUE), rutin (RUT), and control (CON) groups. Rumen fluid was collected at 0, 1, 4, 6, 8, and 22 h, while blood was collected at 0,

SUBMITTER: Aldian D 

PROVIDER: S-EPMC12201991 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature