Archaeal LOV domains from Lake Diamante: first functional characterization of a halo-adapted photoreceptor.

ABSTRACT: High-altitude Andean lakes (HAALs) represent polyextreme environments where diverse photoinduced processes have been documented. In this study, we investigated Light-Oxygen-Voltage (LOV) photoreceptors and identified 28 archaeal sequences from Lake Diamante, which were classified into two major groups (A and B), with three outliers showing unique structural features. Analysis of these sequences and their 3D models revealed hallmark adaptations to halophilic environments, including an abundance of surface acidic residues, an increased prevalence of arginine over lysine, and a greater density of salt bridges. The heterologous expression of a representative LOV domain, ALovD-1, demonstrated conserved photophysics between its dark- and light-adapted states, which was consistent with the slow c

SUBMITTER: Valle L 

PROVIDER: S-EPMC12202551 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature