Genome-wide identification and characterization of soybean GH9 endo-1,4-β-glucanases.

ABSTRACT: Cellulases are a crucial class of enzymes involved in cellulose synthesis and metabolism, significantly contributing to plant growth, development, and organ abscission. The role of Glycosyl hydrolase family 9 (GH9), a major gene family encoding cellulase, remains poorly elucidated in soybean. In this experiment, we identified 43 non-redundant GmGH9 genes in soybean through systematic genome-wide analysis. The physicochemical properties of GmGH9 proteins exhibit variability. Phylogenetic investigations revealed that class B constitutes the predominant evolutionary branch. The GmGH9B/C members display complex splicing patterns. GmGH9As contain typical transmembrane structural domains, while GmGH9Cs uniquely includes the carbohydrate-binding module 49 (CBM49) and signal peptide.

SUBMITTER: Zhan W 

PROVIDER: S-EPMC12202608 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature