ScNucMap: mapping the nucleosome landscapes at single-cell resolution.

ABSTRACT:

Motivation

Nucleosome depletion around cis-regulatory elements (CREs) is associated with CRE activity and implies the underlying gene regulatory network. Single-cell micrococcal nuclease sequencing (scMNase-seq) enables the simultaneous measurement of nucleosome positioning and chromatin accessibility at single-cell resolution, thereby capturing cellular heterogeneity in epigenetic regulation. However, there is currently no computational tool specifically designed to decode scMNase-seq data, impeding the generation of more precise and context-dependent insights into chromatin dynamics and gene regulation.

Results

Here, we present scNucMap, a tool designed to leverage the unique characteristics of scMNase-seq data to map the landscapes of candidate nucleosome-free regions (NF

SUBMITTER: Xiang Q 

PROVIDER: S-EPMC12202765 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature