A Ketol-Acid Reductoisomerase Inhibitor That Has Antituberculosis and Herbicidal Activity.

ABSTRACT: Ketol-acid reductoisomerase (KARI) is a target for the development of new biocidal agents. This is based on its essential role in branched chain amino acid biosynthesis in plants and microorganisms, and its absence in animals. The lack of success in developing KARI inhibitors as biocides may be because the inhibitors assessed to date compete directly with the substrate, 2-acetolactate (AL). As a result, effectiveness diminishes when AL accumulates in cells. Furthermore, as these inhibitors are slow binding, an organism could avoid growth slowdown by increasing KARI production. Here, we show a pyrimidinedione, 1f is a competitive but time-dependent inhibitor of AL and NADPH for Mycobacterium tuberculosis (Mt) KARI (K_i = 23.3 nM). A crystal structure of this compound bound to the

SUBMITTER: Lin X 

PROVIDER: S-EPMC12202844 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature