Tissue-specific DNA isolation from dissected millipedes for nanopore sequencing.

ABSTRACT: There are approximately 12,000 described species within the class Diplopoda. Only five species, falling within 4 of 16 described orders, have fully sequenced genomes. No whole genomes are available for incredibly diverse families like Xystodesmidae. Furthermore, genetic information attributed to key functions in these species is very limited. There is a growing interest in characterizing genomes of non-model organisms, however, extracting high-quality DNA for organisms with complex morphology can be challenging. Here we describe a detailed methodology for obtaining high-purity DNA from legs, head, and body tissues from wild-caught specimens of the millipede species Cherokia georgiana. Our dissection protocol separates the digestive tract minimizing microbial abundance in the extract

SUBMITTER: Cruz E 

PROVIDER: S-EPMC12202871 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature