Molecular Basis of <i>Pseudomonas syringae</i> pv <i>actinidiae</i> Levansucrase Inhibition by a Multivalent Iminosugar.

ABSTRACT: Levansucrases are a class of polysaccharide-processing enzymes widely distributed among plant pathogenic bacteria, such as Pseudomonas syringae and Erwinia amylovora. Therefore, the modulation of levansucrase activity could represent a new strategy to reduce the microbial survival of such bacteria. Herein, we identified a tetravalent pyrrolidine iminosugar (TPIS) as the first levansucrase inhibitor described to date. TPIS reversibly inhibits sucrose hydrolysis and levan polymerization of levansucrase derived from different bacterial genotypes of P. syringae, showing competitive behavior and an inhibition constant (K_i) in the micromolar range. Interestingly, the monovalent pyrrolidine iminosugar (PIS) analogue shows negligible inhibition, suggesting th

SUBMITTER: Cicchi C 

PROVIDER: S-EPMC12203577 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature