Regulatory roles of three-dimensional structures of chromatin domains.

ABSTRACT:

Background

Transcriptional enhancers usually, but not always, regulate genes within the same topologically associating domain (TAD). We hypothesize that this incomplete insulation is partially due to three-dimensional structures of corresponding chromatin domains in individual cells: whereas enhancers and genes buried inside the core of a domain interact mostly with other regions in the same domain, those on the surface can more easily interact with the outside.

Results

Here we show that a simple measure, the intra-TAD ratio, can quantify the coreness of a region with respect to the single-cell domains to which it belongs. We show that domain surfaces are permissive for high gene expression. Cell type-specific active cis-regulatory elements, active histone marks, and transcr

SUBMITTER: Li KY 

PROVIDER: S-EPMC12203726 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature