A Microfluidic Strategy to Capture Antigen-Specific High-Affinity B Cells.

ABSTRACT: Assessing B cell affinity to pathogen-specific antigens prior to or following exposure could facilitate the assessment of immune status. Current standard tools to assess antigen-specific B cell responses focus on equilibrium binding of the secreted antibody in serum. These methods are costly, time-consuming, and assess antibody affinity under zero force. Recent findings indicate that force may influence BCR-antigen binding interactions and thus immune status. Herein, a simple laminar flow microfluidic chamber in which the antigen (hemagglutinin of influenza A) is bound to the chamber surface to assess antigen-specific BCR binding affinity of five hemagglutinin-specific hybridomas from 65 to 650 pN force range is designed. The results demonstrate that both increasing shear force and bound l

SUBMITTER: Alhassan AM 

PROVIDER: S-EPMC12203763 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature