Development and validation of highly sensitive ligand binding assay to measure soluble DLL3 concentration in human serum.

ABSTRACT:

Background

Delta-like protein 3 (DLL3) is considered to inhibit the Notch pathway in the tumorigenesis of small cell lung cancer (SCLC) and other neuroendocrine carcinomas, making it a potential therapeutic target in the treatment of cancer. Since the soluble form (sDLL3) is expected to be useful for predicting the status of DLL3 expression on tumors, analytical methods to measure sDLL3 are required.

Research design and methods

Assay methods using ELISA and the SMCxPRO platform were developed to analyze sDLL3 concentration in human serum. The performance of the ELISA was evaluated and the SMCxPRO assay was fully validated, and the comparability of the 2 assays was assessed.

Results

The performance of the ELISA was acceptable, and in the SMCxPRO assay validation, all p

SUBMITTER: Nishidate M 

PROVIDER: S-EPMC12203847 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature