High-resolution profiling reveals coupled transcriptional and translational regulation of transgenes.

ABSTRACT: Concentrations of RNAs and proteins provide important determinants of cell fate. Robust gene circuit design requires an understanding of how the combined actions of individual genetic components influence both messenger RNA (mRNA) and protein levels. Here, we simultaneously measure mRNA and protein levels in single cells using hybridization chain reaction Flow-FISH (HCR Flow-FISH) for a set of commonly used synthetic promoters. We find that promoters generate differences in both the mRNA abundance and the effective translation rate of these transcripts. Stronger promoters not only transcribe more RNA but also show higher effective translation rates. While the strength of the promoter is largely preserved upon genome integration with identical elements, the choice of polyadenylation signal

SUBMITTER: Peterman EL 

PROVIDER: S-EPMC12203911 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature