A highly sensitive protein-RNA cross-linking mass spectrometry workflow with enhanced structural modeling potential.

ABSTRACT: Protein-RNA interactions underpin many critical biological processes, demanding the development of technologies to precisely characterize their nature and functions. Many such technologies depend upon cross-linking under mild irradiation conditions to stabilize contacts between amino acids and nucleobases; for example, the cross-linking of stable isotope labelled RNA coupled to mass spectrometry (CLIR-MS) method. A deeper understanding of the CLIR-MS workflow is required to maximize its impact for structural biology, particularly addressing the low abundance of cross-linking products and the information content of spatial/geometric restraints reflected by a cross-link. Here, we present a vastly improved CLIR-MS pipeline that features enhanced sample preparation, data acquisition and interp

SUBMITTER: Sarnowski CP 

PROVIDER: S-EPMC12203917 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature