In vitro assessment of berberine-loaded carboxymethyl chitosan hydrogel: A promising antimicrobial candidate for S. aureus-induced bovine mastitis treatment.

ABSTRACT: Bovine mastitis poses significant challenges to the global dairy industry, leading to substantial economic losses and public health concerns. Staphylococcus aureus, a prevalent causative agent of bovine mastitis, depends on effective adhesion and biofilm formation to establish infections. Berberine (BER), a naturally occurring phytochemical, demonstrates broad-spectrum antibacterial activity but suffers from poor bioavailability. This study developed a composite berberine-carboxymethyl chitosan/sodium alginate hydrogel to address these limitations. The hydrogel was characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction. In vitro assessments revealed that the BER hydrogel eradicated S. aureus biofilms (42% eradication at 156.26 μg/mL

SUBMITTER: Wang W 

PROVIDER: S-EPMC12204576 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature