Global siRNA screen identifies human host factors critical for SARS-CoV-2 replication and late stages of infection.

ABSTRACT: Defining the subset of cellular factors governing SARS-CoV-2 replication can provide critical insights into viral pathogenesis and identify targets for host-directed antiviral therapies. While a number of genetic screens have previously reported SARS-CoV-2 host dependency factors, most of these approaches relied on utilizing pooled genome-scale CRISPR libraries, which are biased toward the discovery of host proteins impacting early stages of viral replication. To identify host factors involved throughout the SARS-CoV-2 infectious cycle, we conducted an arrayed genome-scale siRNA screen. Resulting data were integrated with published functional screens and proteomics data to reveal (i) common pathways that were identified in all OMICs datasets-including regulation of Wnt signaling and gap ju

SUBMITTER: Yin X 

PROVIDER: S-EPMC12204624 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature