Catalytic-state structure of Candidatus Hydrogenedentes Cas12b revealed by cryo-EM studies.

ABSTRACT: The CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein) systems are adaptive immune mechanisms that play critical roles in defending archaea and bacteria against invading entities. These systems can be divided into two classes, with class 2 comprising three types (II, V, and VI). Because of their ability to cleave double-stranded DNA, many class 2 CRISPR-Cas proteins have been harnessed as genome editing tools. Unlike the well-studied type II Cas9 proteins, the structural studies of the type V-B Cas12b proteins are limited, hindering their engineering and broader application. Here, we report four complex structures of ChCas12b, which reveal many unique structural features. The folding of the single guide RNA (sgRNA) of ChCas12b is distinct f

SUBMITTER: Li Y 

PROVIDER: S-EPMC12204704 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature