Genome Mining of Fungal Aza-Polycyclic Natural Products Derived from Arginine-Containing Cyclodipeptides.

ABSTRACT: Arginine-containing cyclodipeptide synthases (RCDPSs) from fungi constitute a new family of tRNA-dependent enzymes that can biosynthesize cyclo-Arg-Xaa dipeptides. The incorporation of an arginine residue significantly expands the chemical space of guanidine-containing natural products. Here, we mined fungal biosynthetic gene clusters (BGCs) containing different RCDPS to discover aza-polycyclic natural products. The pno BGC from Aspergillus pseudonomius produced pentacyclic pyrroloindoline diketopiperazines (DKPs) of which the arginine side chain is oxidatively cyclized into a guanidino-proline. Two RCDPS-encoding BGCs, car and esh from Aspergillus carlsbadensis and Eupenicillium shearii, respectively, produced DKPs connected to five- and seven-mem

SUBMITTER: Niwa K 

PROVIDER: S-EPMC12204784 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature