Metabolomic alterations in human brain microvascular endothelial cells induced by traumatic injury.

ABSTRACT:

Introduction

Altered metabolic pathways are critical in the progression of traumatic brain injury (TBI). Identifying differentially abundant metabolites (DAMs) from specific cell types can offer valuable diagnostic and prognostic insights.

Objective

This study aimed to characterize the metabolomic profile of injured human brain microvascular endothelial cells (hBMEC) at 2-, 12-, 24-, and 48 h post-injury.

Methods

Using an in vitro TBI model, we analyzed metabolites in cell culture media through a combination of direct injection mass spectrometry and a custom reverse-phase LC-MS/MS assay. We evaluated 644 metabolites at each time point.

Results

Phosphatidylcholines were significantly upregulated across all time intervals. At 2- and 12 h post-injury, the most sig

SUBMITTER: Cela E 

PROVIDER: S-EPMC12204887 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature