Limited Risk of Microbial Contamination During Hypothermic and Normothermic Machine Perfusion of Donor Kidneys.

ABSTRACT:

Background

Both hypothermic machine perfusion (HMP) and normothermic machine perfusion (NMP) are increasingly used for preservation of deceased donor kidneys. However, especially NMP can pose as a risk for microbial contamination of the kidney graft as the 37°C perfusate can act as a breeding ground for microbial contaminants.

Methods

In this study, we investigated the cultures of HMP and NMP perfusates of deceased donor kidneys.

Results

Between January 2021 and April 2024, a total of 99 perfusates were examined (73 HMP and 26 NMP perfusates)-. We found that contamination of HMP perfusate was common, occurring in 21 of 73 cultures (29%). Most bacteria originated from the skin. Microbial contamination during NMP was rare, occurring only in 2 of 26 cultures (8%). Microb

SUBMITTER: Slagter JS 

PROVIDER: S-EPMC12205300 | biostudies-literature | 2025 May-Jun

REPOSITORIES: biostudies-literature