E3 ubiquitin-protein ligase CBL inhibits non-small cell lung cancer progression via ubiquitination and degradation of KDR.

ABSTRACT:

Background

Non-small cell lung cancer (NSCLC) is the predominant type of lung cancer and continues to be a major cause of cancer-related death globally. The identification of novel molecular targets is essential for developing effective therapeutic strategies.

Methods

In this study, we investigated the functional and mechanistic role of the E3 ubiquitin-protein ligase CBL in NSCLC progression. CBL expression was analyzed in NSCLC tumor tissues and cell lines using quantitative RT-PCR and Western blotting. The impact of CBL on NSCLC cell proliferation, migration, and invasion was assessed by a number of in vitro functional tests. Co-immunoprecipitation (Co-IP) and ubiquitination assays were performed to elucidate the molecular interaction between CBL and kinase insert domain

SUBMITTER: Tu Z 

PROVIDER: S-EPMC12205492 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature