Volume electron microscopy reveals 3D synaptic nanoarchitecture in postmortem human prefrontal cortex.

ABSTRACT: Synaptic function is reflected in quantifiable ultrastructural features using electron microscopy (EM) approaches. This coupling of synaptic function and ultrastructure suggests that in vivo synaptic function can be inferred from EM analysis of ex vivo human brain tissue. To investigate this, we employed focused ion beam-scanning electron microscopy (FIB-SEM), a volume EM (VEM) approach, to generate ultrafine-resolution, three-dimensional (3D) micrographic datasets of postmortem human dorsolateral prefrontal cortex (DLPFC), a region with cytoarchitectonic characteristics distinct to human brain. Synaptic, sub-synaptic, and organelle measures were highly consistent with findings from experimental models that are free from antemortem or postmortem effects. Further, 3D neuropil

SUBMITTER: Glausier JR 

PROVIDER: S-EPMC12205628 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature