IgE clonality and aggregation of insulin affect IgE-mediated activation of sensitized basophils.

ABSTRACT:

Background

Skin prick testing and specific IgE determination do not discriminate between sensitization and clinically relevant insulin allergic reactions, whereas the basophil histamine release assay has proven useful in identifying clinically relevant food and drug allergic reactions.

Objective

We investigated IgE-mediated allergic reactions to insulin by studying the effect of insulin dimerization, superstructures (aggregates), and IgE clonality on activation of sensitized basophils.

Methods

Three humanized IgE molecules directed against distinct insulin epitopes were developed, and basophils, alone or in combination, were sensitized with these antibodies. Insulin in monomeric or dimeric form and superstructures in solution or coupled to a Sepharose matrix were used

SUBMITTER: Christoffersen S 

PROVIDER: S-EPMC12205639 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature