Conservation and diversity of gene families explored using the CODEHOP strategy in higher plants.

ABSTRACT:

Background

Availability of genomewide information on an increasing but still limited number of plants offers the possibility of identifying orthologues, or related genes, in species with major economical impact and complex genomes. In this paper we exploit the recently described CODEHOP primer design and PCR strategy for targeted isolation of homologues in large gene families.

Results

The method was tested with two different objectives. The first was to analyze the evolution of the CYP98 family of cytochrome P450 genes involved in 3-hydroxylation of phenolic compounds and lignification in a broad range of plant species. The second was to isolate an orthologue of the sorghum glucosyl transferase UGT85B1 and to determine the complexity of the UGT85 family in wheat. P450s of th

SUBMITTER: Morant M 

PROVIDER: S-EPMC122057 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature