A BAC-guided haplotype assembly pipeline increases the resolution of the virus resistance locus CMD2 in cassava.

ABSTRACT:

Background

Cassava is an important crop for food security in the tropics where its production is jeopardized by several viral diseases, including the cassava mosaic disease (CMD) which is endemic in Sub-Saharan Africa and the Indian subcontinent. Resistance to CMD is linked to a single dominant locus, namely CMD2. The cassava genome contains highly repetitive regions making the accurate assembly of a reference genome challenging.

Results

In the present study, we generate BAC libraries of the CMD-susceptible cassava cultivar (cv.) 60444 and the CMD-resistant landrace TME3. We subsequently identify and sequence BACs belonging to the CMD2 region in both cultivars using high-accuracy long-read PacBio circular consensus sequencing (ccs) reads. We then sequence and assemble the co

SUBMITTER: Cornet L 

PROVIDER: S-EPMC12206362 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature