A guide to building a low-cost centrifuge force microscope module for single-molecule force experiments.

ABSTRACT: The ability to apply controlled forces to individual molecules or molecular complexes and observe their behaviors has led to many important discoveries in biology. Instruments capable of probing single-molecule forces typically cost >US$100,000, limiting the use of these techniques. The centrifuge force microscope (CFM) is a low-cost and easy-to-use instrument that enables high-throughput single-molecule studies. By combining the imaging capabilities of a microscope with the force application of a centrifuge, the CFM enables the simultaneous probing of hundreds to thousands of single-molecule interactions using tethered particles. Here we present a comprehensive set of instructions for building a CFM module that fits within a commercial benchtop centrifuge. The CFM module uses a 3D-printed

SUBMITTER: Abraham Punnoose J 

PROVIDER: S-EPMC12206937 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature