Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios.

ABSTRACT:

Background

The power of DNA microarrays derives from their ability to monitor the expression levels of many genes in parallel. One of the limitations of such powerful analytical tools is the inability to detect certain transcripts in the target sample because of artifacts caused by background noise or poor hybridization kinetics. The use of base-modified analogs of nucleoside triphosphates has been shown to increase complementary duplex stability in other applications, and here we attempted to enhance microarray hybridization signal across a wide range of sequences and expression levels by incorporating these nucleotides into labeled cRNA targets.

Results

RNA samples containing 2-aminoadenosine showed increases in signal intensity for a majority of the sequences. These resul

SUBMITTER: Nguyen A 

PROVIDER: S-EPMC122072 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature