Enhanced detection of Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) antibodies in various animal species using oligomeric nucleocapsid protein-based diagnostics.

ABSTRACT: Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) is a zoonotic pathogen that affects various animal species, often asymptomatically, while causing fatal disease in humans. Current serological tests are limited, which led to the development of novel diagnostic assays targeting the viral nucleoprotein (NP), a conserved and immunodominant protein across SFTSV strains. This study validated the new diagnostics using field monkey sera, employing Enzyme-Linked Immunosorbent Assay (ELISA) with infected cell lysate (ICL-ELISA) as the antigen. Immunoblotting with recombinant NP (rNP) demonstrated 94.6% specificity and 96.3% sensitivity for anti-NP antibody detection. As NP exists in both monomeric and RNA-binding oligomeric forms, rNP was expressed in both configurations and used for ELISA

SUBMITTER: Chang YC 

PROVIDER: S-EPMC12207807 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature