Identification and potential of the hyperparasite Acremonium persicinum as biocontrol agent against coffee leaf rust.

ABSTRACT: Coffee leaf rust (CLR) caused by Hemileia vastatrix, has emerged as a growing threat to coffee production in China. This study focused on the identification and characterization of the hyperparasitic fungus Acremonium persicinum using integrated plant pathology and molecular biology approaches. The spore suspension of the fungal strain HY85 exhibited a 91.18% inhibition rate against the germination of H. vastatrix urediniospores. Inoculation of coffee leaf discs with H. vastatrix urediniospores resulted in the development of visible chlorotic lesions after 16 days. However, other inoculation methods did not yield early chlorotic lesions, indicating an absence of H. vastatrix infection. Quantitative PCR analysis revealed that the copy numbers of H. vastatrix DNA after 16 days postinoculatio

SUBMITTER: Liang M 

PROVIDER: S-EPMC12207870 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature